Spatial light modulation for interferometric scattering microscopy.

Vivien WalterChristopher ParperisYujie GuoMark Ian Wallace

Published in: Journal of microscopy (2024)

Interferometric scattering (iSCAT) microscopy enables high-speed and label-free detection of individual molecules and small nanoparticles. Here we apply point spread function engineering to provide adaptive control of iSCAT images using spatial light modulation. With this approach, we demonstrate improved dynamic spatial filtering, real-time background subtraction, focus control, and signal modulation based on sample orientation.

Keyphrases

label free
high speed
atomic force microscopy
high resolution
optical coherence tomography
deep learning
single molecule
convolutional neural network
computed tomography
high throughput