Effects of Lactobacillus rhamnosus DSM7133 on Intestinal Porcine Epithelial Cells.

Antimicrobial resistance is one of the biggest health challenges nowadays. Probiotics are promising candidates as feed additives contributing to the health of the gastrointestinal tract. The beneficial effect of probiotics is species/strain specific; the potential benefits need to be individually assessed for each probiotic strain or species. We established a co-culture model, in which gastrointestinal infection was modeled using Escherichia coli ( E. coli ) and Salmonella enterica serovar Typhimurium ( S. enterica serovar Typhimurium). Using intestinal porcine epithelial cells (IPEC-J2), the effects of pre-, co-, and post-treatment with Lactobacillus ( L. ) rhamnosus on the barrier function, intracellular (IC) reactive oxygen species (ROS) production, proinflammatory cytokine (IL-6 and IL-8) response, and adhesion inhibition were tested. E. coli - and S. Typhimurium-induced barrier impairment and increased ROS production could be counteracted using L. rhamnosus ( p < 0.01). S. Typhimurium-induced IL-6 production was reduced via pre-treatment ( p < 0.05) and post-treatment ( p < 0.01); increased IL-8 secretion was decreased via pre-, co-, and post-treatment ( p < 0.01) with L. rhamnosus . L. rhamnosus demonstrated significant inhibition of adhesion for both S. Typhimurium ( p < 0.001) and E. coli ( p < 0.001 in both pre-treatment and post-treatment; p < 0.05 in co-treatment). This study makes a substantial contribution to the understanding of the specific benefits of L. rhamnosus . Our findings can serve as a basis for further in vivo studies carried out in pigs and humans.