Freestanding Nanofiber-Assembled Aptasensor for Precisely and Ultrafast Electrochemical Detection of Alzheimer's Disease Biomarkers.

Amyloid beta-protein (Aβ) is a main hallmark of Alzheimer's disease (AD), and a low amount of Aβ protein accumulation appears to be a potential marker for AD. Here, we developed an electrochemical DNA biosensor based on polyamide/polyaniline carbon nanotubes (PA/PANI-CNTs) with the aim of diagnosing Alzheimer's disease early using a simple, low-cost, and accessible method to rapidly detect Aβ42 in human blood. Electrospun PA nanofibers served as the skeleton for the successive in-situ deposition of PANI and CNTs, which contributed both high conductivity and abundant binding sites for the Aβ42 aptamers. After the aptamers were immobilized, this aptasensor exhibited precise and specific detection of Aβ42 in human blood within only 4 min with an extremely fastest response rate, lower detection limit, and excellent linear detection range. These findings make a significant contribution to advancing the development of serum-based detection techniques for Aβ42, thereby paving the way for improved diagnostic capabilities in the field of AD. This article is protected by copyright. All rights reserved.