A ratiometric fluorescence probe for selective and sensitive detection of leucine aminopeptidase in lysosome.

Di YuanZiwei XuBingling ZhangXiong YinJiqing YeXiaole Zhou Leyu Wang

Published in: Chemical communications (Cambridge, England) (2022)

We designed a novel ratiometric fluorescent probe P1-Leu with a donor-acceptor-donor fluorophore for the detection of leucine aminopeptidase in lysosomes. P1-Leu exhibits a lower detection limit than the ratiometric donor-π-acceptor probe, due to the low ratiometric background. Besides, P1-Leu has good lysosome-targeting ability and realizes the distinction of LAP levels in different cells.

Keyphrases

fluorescent probe
living cells
sensitive detection
loop mediated isothermal amplification
quantum dots
energy transfer
induced apoptosis
single molecule
real time pcr
label free
solar cells
cell cycle arrest
cell proliferation
signaling pathway
cell death
hydrogen peroxide