Soluble overexpression of a flagellin derivative from Salmonella enterica using synonymous codon substitutions of 5'-coding region in Escherichia coli.
Dae-Eun CheongJihye LeeHye-Ji ChoiSu-Kyoung YooDong-Hyun LeeGeun-Joong KimPublished in: Biotechnology letters (2019)
Results showed that scvCBLB502-5, engineered through the synonymous codon substitutions, was easily expressed in functionally soluble form and maintained the proper folding to be recognized by TLR5, as an inducer for pathogen-associated molecular pattern (PAMP).