Detection of colonization capacity of probiotic Bifidobacterium breve CCFM1025 in the human gut.
Xin QianPeijun TianGuopeng LinXinglong XuGang WangHao ZhangWei ChenPublished in: Future microbiology (2023)
Aim: To detect the gut colonization capacity of Bifidobacterium breve CCFM1025 with clinical antidepressant-like effects. Materials & methods: A unique gene sequence of B. breve CCFM1025 was discovered based on the genome analysis of 104 B. breve strains and a strain-specific primer (1025T5) was designed. In vitro and in vivo samples were used to validate the specificity and quantitative capability of this primer in the PCR system. Results: Quantitative PCR using strain-specific primers enabled absolute quantification of CCFM1025 in fecal samples within 10 4 -10 10 cells/g (R 2 >0.99). CCFM1025 remained highly detectable in volunteer feces 14 days after cessation of administration, demonstrating its favorable colonization characteristics. Conclusion: CCFM1025 can colonize the healthy human gut.
Keyphrases
- endothelial cells
- real time pcr
- induced apoptosis
- high resolution
- induced pluripotent stem cells
- genome wide
- cell death
- gene expression
- escherichia coli
- transcription factor
- oxidative stress
- cell cycle arrest
- endoplasmic reticulum stress
- dna methylation
- sensitive detection
- signaling pathway
- quantum dots
- loop mediated isothermal amplification