Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications.
Giuseppe CirilloElvira PantusoManuela CurcioOrazio VittorioAntonella LeggioFrancesca IemmaGiovanni De FilpoFiore Pasquale NicolettaPublished in: Molecules (Basel, Switzerland) (2021)
In this work, we combined electrically-conductive graphene oxide and a sodium alginate-caffeic acid conjugate, acting as a functional element, in an acrylate hydrogel network to obtain multifunctional materials designed to perform multiple tasks in biomedical research. The hybrid material was found to be well tolerated by human fibroblast lung cells (MRC-5) (viability higher than 94%) and able to modify its swelling properties upon application of an external electric field. Release experiments performed using lysozyme as the model drug, showed a pH and electro-responsive behavior, with higher release amounts and rated in physiological vs. acidic pH. Finally, the retainment of the antioxidant properties of caffeic acid upon conjugation and polymerization processes (Trolox equivalent antioxidant capacity values of 1.77 and 1.48, respectively) was used to quench the effect of hydrogen peroxide in a hydrogel-assisted lysozyme crystallization procedure.
Keyphrases
- drug delivery
- hydrogen peroxide
- cancer therapy
- tissue engineering
- wound healing
- induced apoptosis
- hyaluronic acid
- nitric oxide
- drug release
- oxidative stress
- endothelial cells
- cell cycle arrest
- working memory
- minimally invasive
- high speed
- anti inflammatory
- drug induced
- emergency department
- electronic health record
- cell proliferation
- reduced graphene oxide
- cell death
- gold nanoparticles
- extracellular matrix