Planar carbon electrodes for real-time quantification of hydrogen sulfide release from cells.
Jackson R HallJames B TaylorTaron M BradshawMark H SchoenfischPublished in: Sensors & diagnostics (2022)
A planar electrode system was developed to permit the real-time, selective detection of hydrogen sulfide (H 2 S) from stimulated cells. Planar carbon electrodes were produced via stencil printing carbon ink through a laser cut vinyl mask. Electrodes were preconditioned using a constant potential amperometry methodology to prevent sensor drift resulting from elemental sulfur adsorption. Modification with a bilaminar coating (electropolymerized ortho -phenylenediamine and a fluorinated xerogel) facilitated high selectivity to H 2 S. To demonstrate the biological application of this planar sensor system, H 2 S released from 17β-estradiol-stimulated human umbilical vein endothelial cells (HUVECs) was quantified in situ in real-time. Stimulated HUVECs released sustained H 2 S levels for hours before returning to baseline. Cellular viability assays demonstrated negligible cell cytotoxicity at the electrochemical potentials required for analysis.
Keyphrases
- induced apoptosis
- endothelial cells
- cell cycle arrest
- carbon nanotubes
- reduced graphene oxide
- gold nanoparticles
- single cell
- endoplasmic reticulum stress
- label free
- high throughput
- stem cells
- obstructive sleep apnea
- climate change
- high speed
- positive airway pressure
- real time pcr
- aqueous solution
- quantum dots
- human health
- data analysis
- tandem mass spectrometry