Novel economical, accurate, sensitive, single-cell analytical method for mitochondrial DNA quantification in mtDNA mutation carriers.

Weiwei ZouKai ZongZhikang ZhangLingchao ShenXiaolei WangXun SuXin WangTao YinChunmei LiangYajing LiuDan LiangChao HuYunxia CaoDongmei Ji

Published in: Journal of assisted reproduction and genetics (2023)

In conclusion, this study successfully established an economical, accurate, sensitive, single-cell analytical method based on the Sanger sequencing combined ddPCR methods for mtDNA heteroplasmy quantification in a clinical setting.

Keyphrases

mitochondrial dna
single cell
copy number
rna seq
high throughput
high resolution
liquid chromatography
mass spectrometry