Beta adrenergic receptor antagonist can modify Pseudomonas aeruginosa biofilm formation in vitro: Implications for chronic wounds.
Andrea I Medina LopezDaniel R FregosoAnthony GallegosDaniel J YoonJaime J FuentesRobert CrawfordHawa KabaHsin-Ya YangRoslyn Rivkah IsseroffPublished in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022)
Non-healing wounds are a major medical challenge, affecting over 6.5 million people in the US alone, with associated healthcare costs of about $16 billion annually. They can result in prolonged hospitalizations, work loss, disability, poor quality of life, and in diabetic patients with foot ulcers, amputation of the affected limb in 25% of patients. Though chronic ulcers may arise from different underlying diseases, the unifying feature is chronic infection, driving persistent inflammation that prolongs the healing process. One of the most frequently cultured or genetically identified pathogens in skin wounds is Pseudomonas aeruginosa. This species avidly forms biofilms in the wound that impede bacterial eradication by the host's immune mechanisms and limit efficacy of systemic antibiotics. Thus, non-antibiotic approaches to limit the growth and biofilm formation of this wound pathogen would be an advance in the treatment of chronic wounds. Prior work has demonstrated that the growth of other microbial species can be modulated by catecholamine agonists and antagonists of the adrenergic receptors (ARs). Here, we demonstrate that not only can the growth of this common wound pathogen be modulated by catecholamines, but also that the beta-AR antagonists can significantly decrease their growth, and importantly, limit their ability to form biofilms. These findings suggest that beta adrenergic antagonists may have a therapeutic role in the treatment of chronic skin wounds.
Keyphrases
- biofilm formation
- pseudomonas aeruginosa
- wound healing
- candida albicans
- healthcare
- staphylococcus aureus
- cystic fibrosis
- end stage renal disease
- escherichia coli
- type diabetes
- chronic kidney disease
- oxidative stress
- peritoneal dialysis
- acinetobacter baumannii
- ejection fraction
- drug induced
- newly diagnosed
- machine learning
- microbial community
- endothelial cells
- prognostic factors
- helicobacter pylori
- multidrug resistant
- gram negative
- smoking cessation
- health insurance
- patient reported
- affordable care act