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Isolation of phage-display library-derived scFv antibody specific to Listeria monocytogenes by a novel immobilized method.

Xuan-Hung NguyenT-L TrinhT-B-H VuQ-H LeK-A To
Published in: Journal of applied microbiology (2018)
The isolated scFv antibody has potential use in development of immunoassay-based methods for rapid detection of L. monocytogenes in food and environmental samples. In addition, the LECA immobilization method described here could feasibly be employed to isolate specific monoclonal antibodies against any given species of pathogenic bacteria from phage-display libraries.
Keyphrases
  • listeria monocytogenes
  • human health
  • pseudomonas aeruginosa
  • risk assessment
  • cystic fibrosis
  • ionic liquid
  • mass spectrometry
  • label free