Structure of the O-specific polysaccharide of Asaia bogorensis ATCC BAA-21 lipopolysaccharide.

Asaia bogorensis is a Gram-negative bacterium isolated from flowers and fruits growing in tropical climate, reproductive system of mosquitoes, and rarely from immunocompromised patients. In Europe, A. bogorensis is responsible for the contamination of flavoured mineral waters. One of the important surface antigen and an element of the bacterial biofilm is lipopolysaccharide (LPS, endotoxin). To date, no data on A. bogorensis LPS structure has been reported. Chemical analysis and 1 H, 13 C nuclear magnetic resonance spectroscopy revealed the novel structure of the O-specific polysaccharide of A. bogorensis ATCC BAA-21 LPS. It was concluded that the repeating unit of the O-antigen is a branched trisaccharide with the following structure: →6)-α-d-Glcp-(1→2)-[β-d-Glcp-(1→3)]-α-l-Rhap-(1→ .