Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown.

Beob Soo Kim Mitsuru NaitoRimpei KamegawaHyun Jin KimRyo IizukaTakashi FunatsuShingo UenoTakanori IchikiAkihiro KishimuraKanjiro Miyata

Published in: Chemical communications (Cambridge, England) (2021)

A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells.

Keyphrases

induced apoptosis
copy number
genome wide
genome wide identification
electron transfer
cell cycle arrest
endothelial cells
cancer therapy
dna methylation
endoplasmic reticulum stress
cell death
transcription factor
cell proliferation