High-Density Droplet Microarray of Individually Addressable Electrochemical Cells.
Huijie ZhangTobias OellersWenqian FengTarik AbdulazimEn Ning SawAlfred LudwigPavel A LevkinNicolas PlumeréPublished in: Analytical chemistry (2017)
Microarray technology has shown great potential for various types of high-throughput screening applications. The main read-out methods of most microarray platforms, however, are based on optical techniques, limiting the scope of potential applications of such powerful screening technology. Electrochemical methods possess numerous complementary advantages over optical detection methods, including its label-free nature, capability of quantitative monitoring of various reporter molecules, and the ability to not only detect but also address compositions of individual compartments. However, application of electrochemical methods for the purpose of high-throughput screening remains very limited. In this work, we develop a high-density individually addressable electrochemical droplet microarray (eDMA). The eDMA allows for the detection of redox-active reporter molecules irrespective of their electrochemical reversibility in individual nanoliter-sized droplets. Orthogonal band microelectrodes are arranged to form at their intersections an array of three-electrode systems for precise control of the applied potential, which enables direct read-out of the current related to analyte detection. The band microelectrode array is covered with a layer of permeable porous polymethacrylate functionalized with a highly hydrophobic-hydrophilic pattern, forming spatially separated nanoliter-sized droplets on top of each electrochemical cell. Electrochemical characterization of single droplets demonstrates that the underlying electrode system is accessible to redox-active molecules through the hydrophilic polymeric pattern and that the nonwettable hydrophobic boundaries can spatially separate neighboring cells effectively. The eDMA technology opens the possibility to combine the high-throughput biochemical or living cell screenings using the droplet microarray platform with the sequential electrochemical read-out of individual droplets.
Keyphrases
- label free
- high throughput
- high density
- single cell
- gold nanoparticles
- ionic liquid
- molecularly imprinted
- high resolution
- induced apoptosis
- single molecule
- cell therapy
- mass spectrometry
- quantum dots
- stem cells
- cell proliferation
- bioinformatics analysis
- risk assessment
- deep brain stimulation
- liquid chromatography
- drug induced
- drug release