Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene.
Francesco SamboFrancesca FinotelloEnrico LavezzoGiacomo BaruzzoGiulia MasiElektra PetaMarco FaldaStefano ToppoLuisa BarzonBarbara Di CamilloPublished in: BMC bioinformatics (2018)
Results show that our strategy is able to find better primer pairs than the ones available in the literature according to all three optimization criteria. We also experimentally validated three of the primer pairs identified by our method on multiple bacterial species, belonging to different genera and phyla. Results confirm the predicted efficiency and the ability to maximize the number of different bacterial 16S sequences matched by primers.