Login / Signup

Cyclodimerization of Mohangamide A by Thioesterase Domain Is Directed by Substrates.

Myoun-Su KimMunhyung BaeMyoung Chong SongSunghoon HwangDong-Chan OhYeo Joon Yoon
Published in: Organic letters (2022)
Mohangamide A is a pseudo-dimeric nonribosomal peptide biosynthesized along with its monomer, WS9326A, and is expected to be formed by the head-to-tail cyclodimerization of linear WS9326A and another identical peptide chain with a different acyl side chain. In vitro experiments with the N -acetylcysteamine thioesters of the corresponding monomeric intermediates and thioesterase domains of Streptomyces sp. SNM55 and S. calvus showed that this cyclodimerization reaction is directed by the substrate structures and occurs only with both linear intermediates.
Keyphrases
  • high resolution
  • mass spectrometry
  • neural network
  • structural basis
  • tandem mass spectrometry