A Combination of Resistance Genes Confers High and Durable Resistance Against Stripe Rust in Wheat Cultivar Lantian 26.

'Lantian 26', a leading elite winter wheat cultivar in Gansu Province since its release in 2010, exhibits high resistance or immunization to stripe rust in the adult-plant stage under a high disease pressure in Longnan (southeastern Gansu). Identifying the resistance genes in 'Lantian 26' could provide a basis for enhanced durability and high levels of resistance in wheat cultivars. Here, a segregating population was developed from a cross between a highly susceptible wheat cultivar Mingxian 169 and the highly stripe rust-resistant 'Lantian 26'. The F 2 and F 2:3 progenies of the cross were inoculated with multiple prevalent virulent races of stripe rust for adult-plant-stage-resistance evaluation in two different environments. Exon sequence alignment analysis revealed that a stripe rust resistance gene on the 718.4- to 721.2-Mb region of chromosome 7BL, tentatively named as YrLT26 , and a cosegregation sequence-tagged site (STS) marker GY17 was developed and validated using the F 2:3 population and 103 wheat cultivars. The other two resistance genes, Yr9 and Yr30 , were also identified in 'Lantian 26' using molecular markers. Therefore, the key to high and durable resistance to stripe rust at the adult stage is the combination of Yr9 , Yr30 , and YrLT26 genes in 'Lantian 26'. This could be a considerable strategy for improving the wheat cultivars with effective and durable resistance in the high-pressure region for stripe rust.