Performance of a multiplexed amplicon-based next-generation sequencing assay for HLA typing.

Chang LiuBrian F DuffyEric T WeimerMaureen C MontgomeryJo-Ellen JennemannRachel HillDonna PhelanLindsay LayBijal A Parikh

Published in: PloS one (2020)

The AllType assay on the Ion Chef/Ion S5 platform offers a robust and efficient workflow for clinical HLA typing at the 2-field resolution. The multiplex PCR strategy simplifies the laboratory procedure without compromising the typing accuracy.

Keyphrases

high throughput
genetic diversity
single cell
real time pcr
copy number
minimally invasive
gene expression
electronic health record
dna methylation