Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability.

Gianmarco D SuarezDomenick A SuarezYuki Yu Kiu TangJing-Xuan ZhangJun Li Stefan Nagl Peter Pak-Hang Cheung

Published in: Analytical methods : advancing methods and applications (2022)

Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.

Keyphrases

sars cov
loop mediated isothermal amplification
sensitive detection
gold nanoparticles
respiratory syndrome coronavirus
high throughput
hydrogen peroxide
fluorescent probe
aqueous solution
living cells
label free
quantum dots
nitric oxide
coronavirus disease
single molecule