Metabolite profiling and bioactivity of Cicerbita alpina (L.) Wallr. (Asteraceae, Cichorieae).
Dimitrina Zheleva-DimitrovaAlexandra PetrovaMuhammad Imran TousifKouadio Ibrahime SinanVessela BalabanovaOlivier JoubertChristian ZidornYulian VoynikovRumyana SimeonovaReneta GevrenovaPublished in: Plants (Basel, Switzerland) (2023)
Cicerbita alpina (L.) Wallr. is a perennial herbaceous plant in the tribe Cichorieae (Lactuceae), Asteraceae family, distributed in the mountainous regions in Europe. In this study, we focused on the metabolite profiling and the bioactivity of C. alpina leaves and flowering heads methanol-aqueous extracts. The antioxidant activity of extracts, as well as inhibitory potential towards selected enzymes, involving in several human diseases, including metabolic syndrome (α-glucosidase, α-amylase, and lipase), Alzheimer's disease, (cholinesterases: AChE, BchE), hyperpigmentation (tyrosinase), and cytotoxicity were assessed. The workflow comprised ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS). UHPLC-HRMS analysis revealed more than 100 secondary metabolites, including acylquinic, acyltartaric acids, flavonoids, bitter sesquiterpene lactones (STLs), such as lactucin, dihydrolactucin, their derivatives, and coumarins. Leaves showed a stronger antioxidant activity compared to flowering heads, as well as lipase (4.75 ± 0.21 mg OE/g), AchE (1.98 ± 0.02 mg GALAE/g), BchE (0.74 ± 0.06 mg GALAE/g), and tyrosinase (49.87 ± 3.19 mg KAE/g) inhibitory potential. Flowering heads showed the highest activity against α-glucosidase (1.05 ± 0.17 mmol ACAE/g) and α-amylase (0.47 ± 0.03). The obtained results highlighted C. alpina as a rich source of acylquinic, acyltartaric acids, flavonoids, and STLs with significant bioactivity, and therefore the taxon could be considered as a potential candidate for the development of health-promoting applications.
Keyphrases
- high resolution mass spectrometry
- ultra high performance liquid chromatography
- liquid chromatography
- tandem mass spectrometry
- gas chromatography
- ms ms
- metabolic syndrome
- mass spectrometry
- simultaneous determination
- molecular docking
- single cell
- human health
- healthcare
- endothelial cells
- arabidopsis thaliana
- mental health
- electronic health record
- high resolution
- uric acid
- cardiovascular disease
- health information
- type diabetes
- ionic liquid
- skeletal muscle
- risk assessment
- climate change
- social media
- essential oil