Structural Characterization of Terpenoids from Abies holophylla Using Computational and Statistical Methods and Their Biological Activities.
Chung Sub KimJoonseok OhLalita SubediSun Yeou KimSang Un ChoiKang Ro LeePublished in: Journal of natural products (2018)
Three new diterpenoids (1-3) and three new triterpenoids (4-6) were isolated from the trunk of Abies holophylla together with 19 known terpenoids. The chemical structures of 1-6 were determined through NMR and MS data analyses. Also, the structural assignments of some of these compounds were verified and elucidated utilizing computational methods coupled with a statistical procedure (CP3, DP4, and DP4+). All the isolated compounds were evaluated for their cytotoxicity against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15). In addition, the compounds were tested for their anti-inflammatory effects in lipopolysaccharide-stimulated murine microglia BV2 cells by measuring nitric oxide levels, and for their neuroprotective activity in C6 cells through induction of nerve growth factor.
Keyphrases
- growth factor
- cell cycle arrest
- induced apoptosis
- nitric oxide
- inflammatory response
- endothelial cells
- high resolution
- cell death
- mass spectrometry
- magnetic resonance
- lps induced
- multiple sclerosis
- toll like receptor
- endoplasmic reticulum stress
- papillary thyroid
- ms ms
- oxidative stress
- minimally invasive
- big data
- machine learning
- spinal cord injury
- young adults
- nitric oxide synthase