Flow Cytometric Analysis of Erythrocytes Osmotic Fragility in Hereditary Spherocytosis: A Case-Controlled Study Evaluating the Best Anticoagulant, Sample Pre-Treatment and NaCl Concentration for Reliable Screening of this Red Blood Cell Membrane Disorder.

Camila Santos NobreJeovania Almeida SilvaRafael Henriques JácomoLídia Freire Abdalla NeryGustavo Barcelos Barra

Published in: Cytometry. Part B, Clinical cytometry (2018)

EDTA is the anticoagulant of choice for the assay. Incubation at 37°C for 24 h increased its diagnostic capability. The most reliable NaCl concentration for the discrimination of HS case from controls was 6.0 g/L of NaCL in fresh EDTA-treated blood, and was 7.5 g/L of NaCl in incubated EDTA-treated blood. © 2018 International Clinical Cytometry Society.

Keyphrases

atrial fibrillation
venous thromboembolism
single cell
high throughput
newly diagnosed