A novel transgenic mouse strain expressing PKCβII demonstrates expansion of B1 and marginal zone B cell populations.
Ali A AzarAlison M MichieAnuradha TarafdarNatasha MalikGeetha K MenonKathleen J TillNikolina VlatkovićJoseph R SlupskyPublished in: Scientific reports (2020)
Protein kinase Cβ (PKCβ) expressed in mammalian cells as two splice variants, PKCβI and PKCβII, functions in the B cell receptor (BCR) signaling pathway and contributes to B cell development. We investigated the relative role of PKCβII in B cells by generating transgenic mice where expression of the transgene is directed to these cells using the Eµ promoter (Eµ-PKCβIItg). Our findings demonstrate that homozygous Eµ-PKCβIItg mice displayed a shift from IgD+IgMdim toward IgDdimIgM+ B cell populations in spleen, peritoneum and peripheral blood. Closer examination of these tissues revealed respective expansion of marginal zone (MZ)-like B cells (IgD+IgM+CD43negCD21+CD24+), increased populations of B-1 cells (B220+IgDdimIgM+CD43+CD24+CD5+), and higher numbers of immature B cells (IgDdimIgMdimCD21neg) at the expense of mature B cells (IgD+IgM+CD21+). Therefore, the overexpression of PKCβII, which is a phenotypic feature of chronic lymphocytic leukaemia cells, can skew B cell development in mice, most likely as a result of a regulatory influence on BCR signaling.
Keyphrases
- protein kinase
- induced apoptosis
- signaling pathway
- peripheral blood
- transcription factor
- acute lymphoblastic leukemia
- cell cycle arrest
- gene expression
- nk cells
- metabolic syndrome
- endoplasmic reticulum stress
- type diabetes
- cell death
- high fat diet induced
- long non coding rna
- high resolution
- adipose tissue
- atomic force microscopy