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Scaffold Flexibility Controls Binding of Herpes Simplex Virus Type 1 with Sulfated Dendritic Polyglycerol Hydrogels Fabricated by Thiol-Maleimide Click Reaction.

Boonya ThongromAntara SharmaChuanxiong NieElisa QuaasMarwin RaueSumati BhatiaRainer Haag
Published in: Macromolecular bioscience (2022)
Herpes Simplex Virus-1 (HSV-1) with a diameter of 155-240 nm uses electrostatic interactions to bind with the heparan sulfate present on the cell surface to initiate infection. In this work, the initial contact using polysulfate-functionalized hydrogels is aimed to deter. The hydrogels provide a large contact surface area for viral interaction and sulfated hydrogels are good mimics for the native heparan sulfate. In this work, hydrogels of different flexibilities are synthesized, determined by rheology. Gels are prepared within an elastic modulus range of 10-1119 Pa with a mesh size of 80-15 nm, respectively. The virus binding studies carried out with the plaque assay show that the most flexible sulfated hydrogel performs the best in binding HSV viruses. These studies prove that polysulfated hydrogels are a viable option as HSV-1 antiviral compounds. Furthermore, such hydrogel networks are also physically similar to naturally occurring mucus gels and therefore may be used as mucus substitutes.
Keyphrases
  • herpes simplex virus
  • tissue engineering
  • hyaluronic acid
  • drug delivery
  • wound healing
  • drug release
  • extracellular matrix
  • cell surface
  • dna binding
  • high throughput
  • quantum dots
  • single cell
  • genetic diversity