Chemical composition, antifungal and antiproliferative activities of essential oils from Thymus numidicus L.
Ameur ElaissiEman ElsharkawyRidha El MokniHaïfa DebbabiVirginia BrighentiSimona NardoniFederica PellatiSaoussen HammamiPublished in: Natural product research (2020)
In this study, Thymus numidicus Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass spectrometry (GC-MS) and flame ionisation detection (GC-FID) were used for the qualitative analysis and quantification of the volatile constituents. Thymol (50.1-52.8%) was identified as the main compound of both EOs. To evaluate the potential application of the EOs as antifungal agents, the in vitro inhibitory effects were tested against six fungal strains; a strong antifungal activity of one sample was observed (MIC = 40-400 µg/mL). The in vitro antiproliferative activity was investigated on two human cancer cell lines, i.e. the colonic (HCT116) and breast adenocarcinoma (MCF7) using the colourimetric MTT assay. Again, the same sample demonstrated to possess good antiproliferative activity against both cancer cell lines, with IC50 values of 26.9 and 11.7 µg/mL, respectively.
Keyphrases
- gas chromatography
- mass spectrometry
- tandem mass spectrometry
- papillary thyroid
- high resolution mass spectrometry
- gas chromatography mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- candida albicans
- squamous cell
- squamous cell carcinoma
- endothelial cells
- high resolution
- solid phase extraction
- capillary electrophoresis
- escherichia coli
- systematic review
- lymph node metastasis
- south africa
- atomic force microscopy
- simultaneous determination
- childhood cancer
- induced pluripotent stem cells
- radiation therapy
- risk assessment
- breast cancer cells
- ms ms
- cell proliferation
- young adults
- ulcerative colitis
- real time pcr
- tertiary care