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Bright photoactivatable fluorophores for single-molecule imaging.

Jonathan B GrimmBrian P EnglishHeejun ChoiAnand K MuthusamyBrian P MehlPeng DongTimothy A BrownJennifer Lippincott-SchwartzZhe LiuTimothée LionnetLuke D Lavis
Published in: Nature methods (2016)
Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.
Keyphrases
  • single molecule
  • high resolution
  • small molecule
  • atomic force microscopy
  • living cells
  • aqueous solution
  • single cell
  • high throughput
  • cell therapy
  • bone marrow
  • photodynamic therapy
  • highly efficient
  • visible light