Chalcones Bearing Nitrogen-Containing Heterocyclics as Multi-Targeted Inhibitors: Design, Synthesis, Biological Evaluation and Molecular Docking Studies.

In this work, a series of chalcones (1a-d, 2a-d, 3a-d, 4a-d and 5a-d) was designed and synthesized by Claisen-Schmidt condensation. Also, their chemical structures were elucidated using UV-Vis, FT IR, 1 H NMR, 13 C NMR, MS spectral data and Elemental analyses. Subsequently, anticholinesterase, tyrosinase, urease inhibitory activities and antioxidant activities of all chalcones were evaluated. The inhibitory potential of all chalcones in terms of IC 50 value was observed ranging from 7.18 ± 0.43 to 29.62 ± 0.30 μM against BChE by comparing with Galantamine (IC 50 46.06 ± 0.10 μM) as a reference drug. Also compounds 2c, 3c, 4c, 4b and 4d exhibited high anticholinesterase activity against both AChE and BChE enzymes. The tyrosinase inhibitory activity results revealed that three compounds (IC 50 1.75 ± 0.83 μM for 2b, IC 50 2.24 ± 0.11 μM for 3b and IC 50 1.90 ± 0.64 μM for 4b) displayed good inhibitory activity against tyrosinase compared to kojic acid (IC 50 0.64 ± 0.12 μM). In addition, other different three chalcones (IC 50 22.34 ± 0.25 μM for 2c, IC 50 20.98 ± 0.08 μM for 3c and IC 50 18.26 ± 0.13 μM for 4c) showed excellent inhibitory activity against the urease by comparing with thiourea (IC 50 23.08 ± 0.19 μM). Compounds 3c and 4c showed the best potency in all antioxidant activity tests. In the light of these findings, the structure-activity relationship for compounds was also described. Furthermore, molecular modeling studies including molecular docking, ADMET and pharmacophore analyses of compounds gave important information about the interactions and drug likeness properties. As a result, all chalcones exhibited suitable ADMET findings predicting good oral bioavailability. This article is protected by copyright. All rights reserved.