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Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species.

Anamaria Magdalena TomşaAndreea Liana RăchişanStanca Lucia PandreaAndreea BeneaAna UifăleanCorina TomaRoxana PopaAlina Elena PârvuLia Monica Junie
Published in: Metabolites (2022)
Gentamicin remains widely used in all age groups despite its well-documented nephrotoxicity; however, no adjuvant therapies have been established to counteract this side effect. Our study aimed to experimentally determine whether curcumin and vitamin C have nephroprotective effects and whether certain reactive species could be used as markers of early gentamicin nephrotoxicity. Wistar adult male rats were evenly distributed into four groups: control, gentamicin, curcumin and gentamicin, vitamin C and gentamicin (gentamicin: 60 mg/kg/day, intraperitoneally, 7 days). We determined renal function (urea, creatinine), oxidative stress (malondialdehyde, nitric oxide, 3-nitrotyrosine, total oxidative stress), and antioxidant and anti-inflammatory status (thiols, total antioxidant capacity, interleukin-10). Nephrotoxicity was successfully induced, as shown by the elevated creatinine levels in the gentamicin group. In contrast, supplementation with curcumin and vitamin C prevented an increase in urea levels while decreasing total oxidative stress levels compared to the gentamicin group. Moreover, vitamin C and curcumin distinctively modulate the levels of nitric oxide and malondialdehyde. Histological analysis showed more discrete lesions in rats that received vitamin C compared to the curcumin group.
Keyphrases
  • oxidative stress
  • nitric oxide
  • diabetic rats
  • drug induced
  • anti inflammatory
  • dna damage
  • high glucose
  • magnetic resonance
  • metabolic syndrome
  • induced apoptosis
  • uric acid
  • hydrogen peroxide
  • nitric oxide synthase