A high-throughput screening system for SARS-CoV-2 entry inhibition, syncytia formation and cell toxicity.

Shine Varghese JancySanthik Subhasingh LupithaAneesh ChandrasekharanShankara Narayanan VaradarajanShijulal Nelson-SathiRoshny PrasadSara JonesSreekumar EaswaranPramod DarvinAswathy SivasailamThankayyan Retnabai Santhoshkumar

Published in: Biological procedures online (2023)

A BSL-2 compatible assay system that is equivalent to the infectious SARS-CoV-2 is a promising tool for high-throughput screening of large compound libraries for viral entry inhibitors against SARS-CoV-2 along with toxicity and effects on syncytia. Studies using clinical isolates of SARS-CoV-2 are warranted to confirm the antiviral potency of the leads and the utility of the screening system.

Keyphrases

sars cov
respiratory syndrome coronavirus
oxidative stress
single cell
high throughput
mesenchymal stem cells
oxide nanoparticles