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Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis.

Gabriel M C BarrosDionísio D A CarvalhoAgnaldo S CruzEllen K L MoraisAna Isabela Lopes Sales-MoioliTalita Katiane BritoMelise C D AlmeidaIgnacio Sanchez-GendrizFelipe FernandesIngridy Marina Pierre BarbalhoJoão P Q SantosJorge M O HenriquesCésar A D TeixeiraPaulo GilLúcio GamaAngélica E MirandaKarilany D CoutinhoLeonardo J Galvão-LimaRicardo Alexsandro de Medeiros Valentim
Published in: International journal of environmental research and public health (2022)
The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0') or 15 min (T15') after incubation, indicating its potential use as a point-of-care method as a screening strategy.
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