Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera.
Natália Erdens Maron FreitasEmily Ferreira SantosLeonardo Maia LeonyAngelo Antonio Oliveira SilvaRamona Tavares DaltroLarissa de Carvalho Medrado VasconcelosGabriela Agra DuarteCristiane Oliveira da MotaEdimilson Domingos SilvaPaola Alejandra Fiorani CeledonNilson Ivo Tonin ZanchinFred Luciano Neves SantosPublished in: PLoS neglected tropical diseases (2022)
DAgS-ELISA is a promising tool for immunodiagnosis, and despite the high AUC values, the performance of this assay was different from the values obtained by our group when using these antigens in the indirect ELISA, for this reason, improvements are being considered to increase the sensitivity of the DAgS-ELISA.