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Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR.

Mariana Lázaro SalesAntônio Augusto Fonseca JúniorTatiana Flávia Pinheiro de OliveiraAmanda de Araújo LopesAnselmo Vasconcelos Rivetti JúniorMarcelo Fernandes CamargosJenner Karlisson Pimenta Dos Reis
Published in: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2023)
Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the phenotype of VSAV isolates such as in vitro replication fitness. Therefore, the objective of this work was to evaluate the ability of three distinct genotypes of Brazilian isolates of VSAV to grow in different cell-culture lines (BHK-21, Vero, and NCI-H1299). Quantification of viral RNA was performed using RT-PCR digital droplet from supernatant of cell culture collected every 4 h for a period of 24 h of viral growth in three different cell lines (BHK-21, Vero, and NCI-H1299). It was observed that the genotype C isolate has the lowest replication efficiency among the three analyzed viruses, without major changes in the copies of viral RNA over the entire time of the study.
Keyphrases
  • genetic diversity
  • sars cov
  • single cell
  • high throughput
  • physical activity
  • gene expression
  • real time pcr