Imaging of cytotoxic antiviral immunity while considering the 3R principle of animal research.

Lucas OttoGennadiy ZelinskyyMarc SchusterUlf DittmerMatthias Gunzer

Published in: Journal of molecular medicine (Berlin, Germany) (2018)

TCRtg CD8+ T cells are obtained repetitively from the blood samples of single donors. One thousand transferred TCRtg CD8+ T cells get activated, are functional, and proliferate. Several adoptive cell transfers from the same donor show reproducible results. One thousand transferred cells take part in the FV immune response without modifying it. Use of fluorescent transfer cells allows in vivo imaging and single cell tracking.

Keyphrases

single cell
induced apoptosis
immune response
cell cycle arrest
high resolution
cell therapy
rna seq
stem cells
endoplasmic reticulum stress
quantum dots
cell death
high throughput
dendritic cells
cell proliferation
mesenchymal stem cells
pi k akt
toll like receptor
photodynamic therapy