Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton.
Petar PujicNicole AlloisioPascale FournierDavid RocheHaitham SghaierGuylaine MiotelloJean ArmengaudAlison M BerryPhilippe NormandPublished in: Environmental microbiology (2019)
The early Frankia-Alnus symbiotic molecular exchanges were analyzed in detail by protein and RNA omics. For this, Frankia cells were placed in the presence of Alnus roots but separated by a dialysis membrane for 64 h. The bacterial cells were then harvested and analyzed by high-throughput proteomics and transcriptomics (RNA-seq). The most upregulated gene clusters were found to be the potassium transporter operon kdp and an ABC transporter operon of uncharacterized function. The most upregulated proteins were found to be acyl dehydrogenases and the potassium transporter Kdp. These suggest a preadaptation to the impending stresses linked to the penetration into isotonic host tissues and a possible rearrangement of the membrane. Another cluster among the 60 most upregulated ones that comprised two cellulases and a cellulose synthase was conserved among the Frankia and other actinobacteria such as Streptomyces. Cellulase activity was detected on CMC all along the length of the root but not away from it. Frankia alni ACN14a was found to be unable to respire or grow on glucose as sole carbon source. The cellulose synthase was found active at the tip of hyphae in response to Alnus root exudates, resulting in a calcofluor stained tip.
Keyphrases
- single cell
- rna seq
- high throughput
- induced apoptosis
- cell cycle arrest
- chronic kidney disease
- gene expression
- mass spectrometry
- ionic liquid
- endoplasmic reticulum stress
- transcription factor
- single molecule
- end stage renal disease
- adipose tissue
- blood pressure
- insulin resistance
- small molecule
- blood glucose
- silver nanoparticles
- cell proliferation
- aqueous solution
- nucleic acid