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Engineering a better light sheet in an axicon-based system using a flattened Gaussian beam of low order.

Saiedeh SaghafiKlaus BeckerFranco GoriMassih ForoughipourChristine BollweinMeraaj ForoughipourKatja SteigerWilko WeichertHans-Ulrich Dodt
Published in: Journal of biophotonics (2022)
Lasers are fundamental tools in research and development. The shape of an incident laser beam directly affects the results, when it propagates through complex structured meso-aspheric optical elements. In conic-based systems utilizing elements such as axicons, the impact of secondary lobes is mostly overlooked, although the intensity distributions at the central spot and the side-lobes directly affect the beam properties. We investigate the interaction of two axicons (160° and 170°) with incident beams approximated by Gaussian, high-order Flattened-Gaussian, and low-order Flattened-Gaussian functions. We demonstrate that replacing an incident Gaussian beam with a low-order Flattened-Gaussian beam reduces the secondary lobes and significantly improves the uniformity of the intensity profile. We practically applied this effect in engineering a conic-aspheric-based static light-sheet microscope producing markedly improved results.
Keyphrases
  • monte carlo
  • cardiovascular disease
  • electron microscopy
  • high intensity
  • mass spectrometry