Magnetic Nanoscalpel for the Effective Treatment of Ascites Tumors.
Tatiana ZamaySergey ZamayNatalia LuzanVictoriya FedotovskayaAlbert MasyuginFyodor ZelenovAnastasia A KoshmanovaElena NikolaevaDaria KirichenkoDmitry VeprintsevOlga KolovskayaIrina ShchugorevaGalina ZamayIvan N LapinAnna V LukyanenkoAndrey BorusAlexander SukhachevMikhail VolochaevKirill A LukyanenkoAlexandr ShabanovVladimir ZabludaAlexey Y ZhizhchenkoAleksandr KuchmizhakAlexey E SokolovAndrey NarodovVladimir ProkopenkoRinat GaleevValery A SvetlichnyiAnna KichkailoPublished in: Journal of functional biomaterials (2023)
One of the promising novel methods for radical tumor resection at a single-cell level is magneto-mechanical microsurgery (MMM) with magnetic nano- or microdisks modified with cancer-recognizing molecules. A low-frequency alternating magnetic field (AMF) remotely drives and controls the procedure. Here, we present characterization and application of magnetic nanodisks (MNDs) as a surgical instrument ("smart nanoscalpel") at a single-cell level. MNDs with a quasi-dipole three-layer structure (Au/Ni/Au) and DNA aptamer AS42 (AS42-MNDs) on the surface converted magnetic moment into mechanical and destroyed tumor cells. The effectiveness of MMM was analyzed on Ehrlich ascites carcinoma (EAC) cells in vitro and in vivo using sine and square-shaped AMF with frequencies from 1 to 50 Hz with 0.1 to 1 duty-cycle parameters. MMM with the "Nanoscalpel" in a sine-shaped 20 Hz AMF, a rectangular-shaped 10 Hz AMF, and a 0.5 duty cycle was the most effective. A sine-shaped field caused apoptosis, whereas a rectangular-shaped field caused necrosis. Four sessions of MMM with AS42-MNDs significantly reduced the number of cells in the tumor. In contrast, ascites tumors continued to grow in groups of mice and mice treated with MNDs with nonspecific oligonucleotide NO-MND. Thus, applying a "smart nanoscalpel" is practical for the microsurgery of malignant neoplasms.
Keyphrases
- cell cycle arrest
- single cell
- induced apoptosis
- molecularly imprinted
- cell free
- endoplasmic reticulum stress
- cell death
- rna seq
- sensitive detection
- oxidative stress
- systematic review
- magnetic resonance
- pi k akt
- gold nanoparticles
- high fat diet induced
- adipose tissue
- signaling pathway
- minimally invasive
- papillary thyroid
- single molecule
- mass spectrometry