A novel plasmid-Escherichia coli system produces large batch dsRNAs for insect gene silencing.

Zhong-Zheng MaHang ZhouYan-Long WeiShuo YanJie Shen

Published in: Pest management science (2020)

Our system expressed dsRNA more efficiently than the widely used L4440-HT115(DE3) system, and the produced dsRNA showed a high gene-silencing effect. Notably, our pET28-BL21(DE3) RNase III-system provides a novel method for the mass production of dsRNA at low cost and high efficiency, which may promote gene function analysis and RNAi-based pest management. © 2020 Society of Chemical Industry.

Keyphrases

low cost
escherichia coli
high efficiency
computed tomography
copy number
crispr cas
positron emission tomography
genome wide
genome wide identification
multidrug resistant
aedes aegypti
transcription factor