High activity CAZyme cassette for improving biomass degradation in thermophiles.
Roman BruneckyDaehwan ChungNicholas S SaraiNeal HenggeJordan F RussellJenna YoungAshutosh MittalPatthra PasonTodd Vander WallWilliam MichenerTodd ShollenbergerJanet WestphelingMichael E HimmelYannick J BomblePublished in: Biotechnology for biofuels (2018)
We have demonstrated that it is possible to replicate the cellulolytic activity of the native C. bescii exoproteome utilizing a minimal gene set, and that these minimal gene sets are more active than the whole exoproteome. In the future, this may lead to more simplified and efficient cellulolytic enzyme preparations or yield improvements when these enzymes are expressed in microorganisms engineered for consolidated bioprocessing.