Recombinant production of the lantibiotic nisin using Corynebacterium glutamicum in a two-step process.
Dominik WeixlerMax BerghoffKirill V OvchinnikovSebastian ReichOliver GoldbeckGerd M SeiboldChristoph WittmannNadav S BarBernhard J EikmannsDzung B DiepChristian U RiedelPublished in: Microbial cell factories (2022)
We demonstrate successful implementation of a two-step process for recombinant production of active nisin with C. glutamicum. This extends the spectrum of bioactive compounds that may be produced using C. glutamicum to a bacteriocin harboring complex posttranslational modifications. Our results provide a basis for further studies to optimize product yields, transfer production to sustainable substrates and purification of pharmaceutical grade nisin.
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