Building a reference transcriptome for Juniperus squamata (Cupressaceae) based on single-molecule real-time sequencing.
Yufei WangSiyu XieJialiang LiJieshi TangTsam JuKang-Shan MaoPublished in: BMC genomic data (2021)
Juniperus squamata, a tetraploid species which is widely distributed in Asian mountains, represents the largest genus, Juniperus, in Cupressaceae. Single-molecule real-time sequencing was used to obtain full-length transcriptome of Juniperus squamata. The full-length transcriptome was corrected with Illumina RNA-seq data from the same individual. A total of 47,860 non-redundant full-length transcripts, N50 of which was 2839, were obtained. A total of 57,393 simple sequence repeats were identified and 268,854 open reading frames were predicted for Juniperus squamata. A BLAST alignment against non-redundant protein database was conducted and 10,818 sequences were annotated in Gene Ontology database. InterPro analysis shows that 30,403 sequences have been functionally characterized against its member database. This data presents the first comprehensive transcriptome characterization of Juniperus species, and provides an important reference for researches on the genomics and evolutionary history of Cupressaceae plants and conifers in the future.
Keyphrases
- single cell
- rna seq
- single molecule
- atomic force microscopy
- genome wide
- living cells
- electronic health record
- adverse drug
- gene expression
- big data
- working memory
- minimally invasive
- genetic diversity
- emergency department
- machine learning
- amino acid
- data analysis
- small molecule
- deep learning
- drug induced
- fluorescent probe