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O-GlcNAcylation regulates lysophosphatidic acid-induced cell migration by regulating ERM family proteins.

Minseok SongPann-Ghill Suh
Published in: FEBS open bio (2022)
O-GlcNAcylation of intracellular proteins (O-GlcNAc) is a post-translational modification that often competes with phosphorylation in diverse cellular signaling pathways. Recent studies on human malignant tumors have demonstrated that O-GlcNAc is implicated in cellular features relevant to metastasis. Here, we report that lysophosphatidic acid (LPA)-induced ovarian cancer cell (OVCAR-3) migration is regulated by O-GlcNAc. We found that O-GlcNAc modification of ERM family proteins, a membrane-cytoskeletal crosslinker, was inversely correlated with its phosphorylation status. Moreover, the LPA-induced formation of membrane protrusion structures, as well as the migration of OVCAR-3 cells, was reduced by the accumulation of O-GlcNAc. Collectively, these findings suggest that O-GlcNAc is an essential signaling element controlling ERM family proteins involved in OVCAR-3 cell migration.
Keyphrases
  • cell migration
  • high glucose
  • endothelial cells
  • induced apoptosis
  • signaling pathway
  • diabetic rats
  • drug induced
  • cell cycle arrest
  • pi k akt
  • mass spectrometry
  • induced pluripotent stem cells
  • cell proliferation