TEM-184, a Novel TEM-Derived Extended-Spectrum β-Lactamase with Enhanced Activity against Aztreonam.

Alessandra PiccirilliMariagrazia PerilliGianfranco AmicosanteViola ConteCarlo TasciniGian Maria RossoliniTommaso Giani

Published in: Antimicrobial agents and chemotherapy (2018)

TEM-184, a novel TEM-derived extended-spectrum β-lactamase (ESBL), was isolated from an Escherichia coli ST354 clinical strain. Compared to TEM-1, TEM-184 contains the mutations Q6K, E104K, I127V, R164S, and M182T. Kinetic analysis of this enzyme revealed extended-spectrum activity against aztreonam in particular. TEM-184 was also susceptible to inhibitors, including clavulanic acid, tazobactam, and avibactam.

Keyphrases

escherichia coli
klebsiella pneumoniae
gram negative
multidrug resistant
biofilm formation
cystic fibrosis