De novo synthesis of alkynalted tryptophan moieties as chemical probes for protein profilling studies, via an unexpected synthesis of Michael acceptor 12 is reported. Friedel Craft's alkylation of 12 and alkyne substituted indoles gave alkynalated tryptophan moieties, which perform as chemical probe by incorporating into actively translating Escherichia coli cells, whereby the alkyne moiety enables multimodal analyses through click chemistry mediated attachment of reporting groups.
Keyphrases
- escherichia coli
- living cells
- small molecule
- molecular docking
- induced apoptosis
- protein protein
- fluorescence imaging
- case control
- emergency department
- binding protein
- amino acid
- oxidative stress
- cell cycle arrest
- single cell
- quantum dots
- chronic pain
- pain management
- adverse drug
- klebsiella pneumoniae
- candida albicans
- nucleic acid
- pseudomonas aeruginosa