Eucheuma cottonii Sulfated Oligosaccharides Decrease Food Allergic Responses in Animal Models by Up-regulating Regulatory T (Treg) Cells.
Sha-Sha XuQing-Mei LiuAn-Feng XiaoSoheila J MalekiMarcos AlcocerYuan-Yuan GaoMin-Jie CaoGuang-Ming LiuPublished in: Journal of agricultural and food chemistry (2017)
In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77.7%. In addition, the production of interleukin (IL)-4 and IL-13 was diminished in the ESO groups compared to the non-ESO-treated group. Furthermore, ESO could up-regulate Treg cells by 22.2-97.1%. In conclusion, ESO decreased the allergy response in mice by reducing basophil degranulation, up-regulating Treg cells via Forkhead box protein 3 (Foxp3), and releasing IL-10. ESO may have preventive and therapeutic potential in allergic disease.
Keyphrases
- induced apoptosis
- cell cycle arrest
- mouse model
- transcription factor
- endoplasmic reticulum stress
- type diabetes
- flow cytometry
- dendritic cells
- risk assessment
- skeletal muscle
- small molecule
- regulatory t cells
- adipose tissue
- high throughput
- cell proliferation
- protein protein
- liquid chromatography
- high fat diet induced
- newly diagnosed