INSIGHT: A population-scale COVID-19 testing strategy combining point-of-care diagnosis with centralized high-throughput sequencing.
Qianxin WuChenqu SuoTom BrownTengyao WangSarah A TeichmannAndrew R BassettPublished in: Science advances (2021)
We present INSIGHT [isothermal NASBA (nucleic acid sequence-based amplification) sequencing-based high-throughput test], a two-stage coronavirus disease 2019 testing strategy, using a barcoded isothermal NASBA reaction. It combines point-of-care diagnosis with next-generation sequencing, aiming to achieve population-scale testing. Stage 1 allows a quick decentralized readout for early isolation of presymptomatic or asymptomatic patients. It gives results within 1 to 2 hours, using either fluorescence detection or a lateral flow readout, while simultaneously incorporating sample-specific barcodes. The same reaction products from potentially hundreds of thousands of samples can then be pooled and used in a highly multiplexed sequencing-based assay in stage 2. This second stage confirms the near-patient testing results and facilitates centralized data collection. The 95% limit of detection is <50 copies of viral RNA per reaction. INSIGHT is suitable for further development into a rapid home-based, point-of-care assay and is potentially scalable to the population level.
Keyphrases
- nucleic acid
- high throughput
- coronavirus disease
- single cell
- loop mediated isothermal amplification
- sars cov
- end stage renal disease
- chronic kidney disease
- ejection fraction
- high throughput sequencing
- label free
- prognostic factors
- real time pcr
- respiratory syndrome coronavirus
- machine learning
- electronic health record
- amino acid
- single molecule
- quantum dots