Molecular docking and nucleotide sequencing of successive expressed recombinant fungal peroxidase gene in E.coli.
Mohamed KhedrKamal M A KhalilHoda A KabaryAhmed A HamedMona Shaban E M BadawyMohammed Abu-ElghaitPublished in: Journal, genetic engineering & biotechnology (2022)
A successful peroxidase-gene (PER-K) transformation from fungi to bacteria was performed correctly. The enzyme activity was screened, and partial sequencing of PER-K gene was analyzed successively. The protein 3D structure was generated via in silico homology modeling, and determination of binding sites and biological annotations of the constructed protein were carried out via COACH and COFACTOR based on the I-TASSER structure prediction.