AChE mRNA expression as a possible novel biomarker for the diagnosis of coronary artery disease and Alzheimer's disease, and its association with oxidative stress.
Mesut IşıkSukru BeydemirPublished in: Archives of physiology and biochemistry (2019)
Oxidative metabolic reactions and their by products have played a role in coronary artery disease (CAD) and Alzheimer's disease (AD) pathogenesis. This study was carried out on 28 patients with AD, 21 patients with CAD, and 28 healthy as control. Oxidative stress biomarkers and acetylcholinesterase (AChE) activity were assayed in plasma. mRNA expression of AChE was investigated in leukocytes of patients with CAD and AD. Thus, Alzheimer's and coronary artery patients were observed that the protein carbonyl levels and mRNA expression of AChE were increased (p<.05, p<.01, respectively). The plasma total thiol levels were decreased compared to the control group (p<.05). There was a significant relationship between amyloid β (Aβ) accumulation and oxidative stress, cholinergic gene expression. AChE gene expression and protein oxidation were increased in patients with AD and CAD. These results suggest that increased release of AChE from cells produces neurotoxic β-amyloid plaques and may cause neurodegenerative diseases.
Keyphrases
- coronary artery disease
- oxidative stress
- gene expression
- induced apoptosis
- percutaneous coronary intervention
- coronary artery
- cardiovascular events
- coronary artery bypass grafting
- cognitive decline
- dna damage
- dna methylation
- ischemia reperfusion injury
- ejection fraction
- diabetic rats
- newly diagnosed
- endoplasmic reticulum stress
- pulmonary artery
- protein protein
- hydrogen peroxide
- peripheral blood
- cell cycle arrest
- binding protein
- mass spectrometry
- acute coronary syndrome
- cell death
- patient reported
- pulmonary hypertension
- atomic force microscopy
- aortic valve
- transcatheter aortic valve replacement