Microenvironment-Responsive Small-Molecule Probe for Pulmonary Fibrosis Detection.
Ying DongXiao-Rong LiQi ChenRui-Ying GuoBi-Xi TangWei-Juan KanWei ZhangYongzhou HuJia LiYi ZangXin LiPublished in: Analytical chemistry (2019)
Pulmonary fibrosis (PF) is a fatal disease with increasing prevalence. Nonradioactive and noninvasive diagnosis of PF at an early stage can improve the prognosis but represents a daunting challenge. Up-regulation of nitric oxide (NO) is a typical microenvironmental feature of PF. Here, we report a small-molecule probe, PNO1, that can fluorogenically sense this microenvironmental feature for PF diagnosis. We demonstrate that PNO1 fluorescence is 6-fold higher in PF-diseased mice lungs than in normal-control groups. In addition to this in vivo result, PNO1 can also be applied in vitro to detect PF-diseased cells and ex vivo to detect PF-diseased tissues from clinical patients. These results highlight PNO1 as a complement to the traditional immunostaining-based methods for PF detection to facilitate quick screening for anti-PF drug candidates.
Keyphrases
- small molecule
- pulmonary fibrosis
- nitric oxide
- early stage
- stem cells
- machine learning
- gene expression
- newly diagnosed
- induced apoptosis
- end stage renal disease
- ejection fraction
- type diabetes
- hydrogen peroxide
- risk factors
- chronic kidney disease
- drug delivery
- quantum dots
- oxidative stress
- neoadjuvant chemotherapy
- nitric oxide synthase
- skeletal muscle
- insulin resistance
- locally advanced
- endoplasmic reticulum stress
- wild type