Neuroprotective Activity of a Novel Synthetic Rhodamine-Based Hydrazone against Cu 2+ -Induced Alzheimer's Disease in Drosophila .

A new rhodamine-based probe 3,5-di- tert -butylsalicylaldehyde rhodamine hydrazone ( RHTB ) has been synthesized and well characterized using spectroscopic techniques and single-crystal X-ray crystallography. Among several metal ions, it selectively detects Cu 2+ ions as monitored by UV-Vis and emission spectral titrations. It displays "turn on" behavior owing to the opening of a spirolactum ring and the presence of 3,5-di- tert -butyl as an electron releasing group. Further, Cu 2+ ions play a pivotal role in extracellular aggregation of Aβ 42 peptides. So far, we know probably that there are no promising drugs available in this regard. Hence, countering the Cu 2+ ions by RHTB chelation against orally administered Cu 2+ ion-induced neurotoxicity in the eye tissue of Drosophila expressing human Aβ 42 (amyloid-β 42 ) has been tested. The present study involves in vivo and in silico approaches. They reveal the therapeutic potential of RHTB against Cu 2+ ion-induced Aβ 42 toxicity in Alzheimer's disease (AD) model of Drosophila .