Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype.

Benjamin Lopez-JimenaMichaël BekaertMohammed BakheitSieghard FrischmannPranav PatelEtienne Simon-LoriereLouis LambrechtsVeasna DuongPhilippe DussartGraham HaroldCheikh FallOumar FayeAmadou Alpha SallManfred Weidmann

Published in: PLoS neglected tropical diseases (2018)

We have shown a novel approach to design LAMP primers that makes use of fast growing sequence databases. The DENV1 and DENV2 assays were validated with viral RNA extracted clinical samples, showing very good performance parameters.

Keyphrases

dengue virus
loop mediated isothermal amplification
zika virus
high throughput
sensitive detection
aedes aegypti
sars cov
big data
escherichia coli
amino acid
artificial intelligence
multidrug resistant